The "mother queen's" daughter

This weekend I checked the nucs to see if the queens had mated. Neither of the two miller frame queen cells were accepted by the nucs. At least the queens were no where to be found.

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One of the two queen cells produced from my first attempt at grafting. 2 out of 30 cells were accepted.

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It was obvious that the queens had emerged from the queen cells, but the queens did not return from their mating flights. I did find one emergency queen cell in one of the mating nucs. These bees must have rejected the queen in favor of one that they are raising themselves.

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My first mated and laying queen. She is the only daughter from my best queen who went missing a few weeks ago. This daughter will be introduced into her mothers hive.

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Of the 30 grafted queen cups placed in the cell builder hive, only two queen cells were produced. That is less than a 10% acceptance rate. This is not as good as I expected, even for a first try at grafting. Since the queen cells were capped I placed them in cell protectors and introduced them into mating nucs consisting of two deep frames of bees each. These grafted queen cells are scheduled to emerge on July 4th or 5th.

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A frame of eggs and larva. Newly hatched larva was taken from this frame and grafted into queen cell cups.

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One happy discovery was to find a mated queen in the third mating nuc. This queen was raised by the bees from an egg from the mother queen which has since gone missing. Since she is the only daughter produced by my favorite queen, I am introducing her into her mothers hive (which is still queenless, and now broodless). With in a few days she should be accepted by the hive and be laying. To introduce her I placed the frames of bees from the nuc into a deep hive body. The hive body was then placed over two sheets of news paper above the queenless colony. The bees will chew a hole in the newspaper within a day or so. By that time the new queens scent will be distributed in the hive and with any luck the bees will accept her as their new queen.

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90 grafted queen cups

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Since the cell builder hive is now without any queen cells, I added two frames of brood an bees, as well as two grafted frames holding 90 grafted cell cups. If I only have a 10% acceptance rate I should end up with at least 9 queen cells if not more.

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A closeup of the grafted cells. You can see the very young larva floating on a drop of royal jelly

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48 hours will tell.